Qualities of a Good Vector

Q. Assertion: The bacterial cells must first be made ‘competent’ to take up foreign DNA.
Reason: DNA cannot pass through cell membranes since it is a hydrophilic molecule.
Select the correct option.

1. Both the assertion and reason are true and the reason is the correct explanation of assertion
2. Both the assertion and reason are true and reason is not the correct explanation of assertion
3. Assertion is true but the reason is false
4. Both the assertion and reason are false

Q.

What are cloning sites and explain their role?

Q. What occurs when a $F^{+}$ bacterium conjugates with $F^{–}$ bacterium?

1. $F^{+}$ gets modified into $F^{–}$
2. $F^{–}$ gets modified into $F^{+}$
3. $F^{–}$ gets modified into $F^{–}$
4. Hfr gets modified into $F^{-}$

Q.

Do Prokaryotes have multiple origins of replication?

Q. Assertion (A): DNA ligase plays an essential role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.

1. Both A and R are true and R is the correct explanation of A
2. Both A and R are true but R is not the correct explanation of A
3. A is true but R is false
4. Both A and R are false

Q. A scientist tried to transform bacteria with a plasmid containing the gene of interest inserted within the lacZ gene. Few of the bacteria took up the plasmid while many didn’t. He plated this mixture of bacteria on a medium containing X-gal. Choose the illustration which correctly represents the results he would have obtained based on blue-white screening.

1. 
2. 
3. 
4. 

Q. Cohen and Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid having antibiotic resistance in the year

1. 1963
2. 1967
3. 1972
4. 1982

Q. Find the mismatch

1. pBR322 - artificially synthesised plasmid
2. YAC - Host organism
3. BAC - bacterial artificial chromosome
4. pUC19 - artificially synthesised plasmid

Q. Which of the following features is not necessary for cloning vectors?

1. Origin of replication
2. High copy number
3. Selectable marker
4. Cloning sites

Q. Following are the two statements regarding PCR primers.

Statement1: PCR primers must not have self-complementary regions.
Statement 2: Self-complementary regions result in hairpin structures, adversely affecting the PCR.

1. Both the statements are true
2. Statement 1 is true and statement 2 is false
3. Statement 1 is false and statement 2 is true
4. Both the statements are false

Q.

What is meant by cloning vector?

Q.

How is foreign DNA inserted?

Q. Cells are made competent by a process that uses

1. calcium chloride and micro injection
2. potassium chloride and heat shock
3. calcium chloride and heat shock
4. potassium chloride and microinjection

Q. The alphabets ‘B’ and ‘R’ in the plasmid pBR322 represent which of the following?

1. Names of the scientists who developed the plasmid
2. Name of the restriction endonuclease enzymes
3. Name of University where vector was developed
4. Strain of bacteria

Q. How is the copy number of the plasmid vector related to yield of recombinant protein?

Q. Write the role of 'Ori' and 'restriction' site in a cloning vector pBR322.

Q. Explain the features of cloning vectors.

Q. Which of the following is not required in the preparation of a recombinant DNA molecules?

1. Restriction endonucleases
2. DNA ligase
3. DNA fragments
4. E. coli

Q. Which type of plasmid contains antibiotic resistance gene?

1. "R"- factor
2. "S"- factor
3. "G"- factor
4. "P"- factor

Q. Read the given statements and select the correct option.
Statement 1 : The cloning vector is required to have very few, preferably single, recognition sites for the commonly used restriction enzymes.Statement 2 : Presence of more than one recognition sites within a cloning vector will generate several fragments, which will complicate the process of gene cloning.

1. Both statements 1 and 2 are correct
2. Statement 1 is correct but statement 2 is incorrect
3. Statement 1 is incorrect but statement 2 is correct
4. Both statements 1 and 2 are incorrect

Q. What is the role of Ori for cloning vector?

Q. Plasmids are used in genetic engineering because they are

1. Easily available
2. Able to replicate
3. Able to integrate with host chromosome
4. Inert

Q. A scientist synthesised a new plasmid for her research work. Her intention was to insert a human gene into the plasmid and harvest large amounts of protein. But in spite of several rounds of experimentation, she found that these bacteria did not produce the protein of choice. What could be the problem with the plasmid?

1. Transgene is not inserted in the correct position in plasmid
2. The plasmid does not have the transgene
3. The plasmid does not have a marker
4. The ori site is missing

Q. A person trying to create a recombinant DNA molecule failed to do so. Below is the procedure that he followed.

Which among the following is the most probable mistake he might have made?

1. He did not use the enzyme DNA polymerase
2. He used double stranded mammalian DNA
3. He used two different restriction enzymes
4. He inserted only one gene of the mammalian DNA into the plasmid

Q. Markers are usually __ genes.

Q.

is pBR322 the first artificial cloning vector to be constructed ?

what does BR stands for ?

Q.

if a protein incoding gene is expressed in a heterologous host, it is calles recombinant protein.

what does this mean?

Q. In genetic engineering, the antibiotics are used

1. To select healthy vectors
2. As selectable markers
3. As sequence from where replication starts
4. To keep the cultures free of infection

Q. What is the role of markers in a plasmid?

1. To help in selecting the cells that are transformed
2. To mark the proteins synthesized by the gene of interest
3. To mark the location from where the replication starts
4. To mark the location of transgene in the plasmid

Q.
Q. "Therefore, for the multiplication of any alien piece of DNA in an organisms it needs to be a part of a chromosome(s) which has a specific sequences known as origin of replication. " Explain the above lines.
Wont all chromosomes have their own origin of replication?