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Question

DNA is digested with a restriction endonuclease and subjected to gel electrophoresis.
Choose the correct statement w.r.t. to the above, among the following:

A
DNA fragments will migrate towards the cathode
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B
Number of bands equals the number of restriction sites within the DNA
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C
DNA fragments will migrate towards the anode
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D
There is no correlation between the number of bands on the agarose gel and restriction sites in the DNA
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Solution

The correct option is C DNA fragments will migrate towards the anode
During electrophoresis, DNA migrates towards the positive terminal (anode) because DNA is a negatively charged molecule and its phosphate group is located at the backbone.
The number of fragments of DNA/number of bands on the gel formed are correlated to the number of restriction sites for the enzyme in the DNA by the formula "n+1", where "n" is the number of restriction sites.

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