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Byju's Answer
Standard XII
Biology
Ligase
During gene c...
Question
During gene cloning, the enzyme used to join and insert DNA within plasmid is
A
DNA ligase
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B
Restriction endonuclease
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C
Alkaline phosphatase
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D
Exonuclease
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Solution
The correct option is
A
DNA ligase
So the correct option is A.
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Similar questions
Q.
During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is
Q.
Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant
molecule?
Q.
Plasmid pBR322 has Pst I restriction enzyme site within gene amp
R
that confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain
Q.
Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is
true about restriction enzymes except
Q.
Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?
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