Question

How probe sequence is determined in autoradiography molecular diagnosis

Answer 1

Dear student,
In case of autoradiography, probe sequences are determined by application of radiolabeled ligands in the sections in inspection. Usually ligands are marked with ³H or ¹²⁵I isotopes are used, although in cases of RNA or DNA viral sequences, ³²P, ³³P or ³⁵S probes are also useed. Via marking and detecting the radioactive emissions from the probes attached, the sequence patterns and the functional patterns are detected.

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