Question

_______test is based on the principle of antigen-antibody interaction.

• A. DNA fingerprinting
• B. ELISA
• C. PCR
• D. All of these

Answer 1

The correct option is B ELISA

ELISA is a basic assay technique, known as enzyme-linked immunosorbent assay, that is carried out to detect and measure antibodies, antigens, hormones, peptides and proteins in the blood.

ELISA works on the principle of antibody-antigen interaction. Antibody is a protein produced by the immune system in response to the presence of a foreign substance, antigen.

Antibodies recognise and bind to the antigens in order to remove them from the body. This interaction between antigen and antibody is utilized in ELISA testing and allows for identifying specific antibodies and antigens.

PCR, polymerase chain reaction, is a method widely used to rapidly make millions of copies of a specific DNA sequence of desired samples. PCR test helps in the early diagnosis of certain pathogens like the HIV.

Sir Alec Jeffreys developed a method of DNA fingerprinting. This method involves identifying a person based on certain repetitive sequences in the DNA.