The sequence of strategies involved in the isolation of a gene of interest from a plant cell during the process of rDNA technology
The sequence of strategies involved in the isolation of a gene of interest from a plant cell during the process of rDNA technology
The correct option is C Precipitation of DNA
Isolation of the Genetic Material (DNA):
Nucleic acid is the genetic material, which is present in
all living organisms. In majority of organisms, this is present in the form of
deoxy-ribonucleic acid (DNA). DNA must be present into pure form, i.e., free
from other macro-molecules (like proteins, RNA, enzymes, etc.) in order to cut
the DNA with restrictor enzymes.
Isolation
of genetic material (DNA) is carried out in the following steps:
(a) Since the DNA is enclosed within
the membranes, so, in order to release DNA along with other macro-molecules
such as proteins, polysaccharides and lipids, bacterial cells/plant or animal
tissues are treated with the enzyme lysozyme (bacteria), cellulose (plant
cells), chitinase (fungus), respectively.
(b) RNA can be removed by
the treatment with ribonuclease, whereas proteins can be removed by the
treatment with protease.
(c) Other molecules can
be removed by appropriate treatments and ultimately purified DNA will
precipitate out, after the addition of chilled ethanol. This can be seen as
collection of fine threads in the suspension.
So, the correct answer is “Precipitation
of DNA”.
Isolation of the Genetic Material (DNA):
Nucleic acid is the genetic material, which is present in all living organisms. In majority of organisms, this is present in the form of deoxy-ribonucleic acid (DNA). DNA must be present into pure form, i.e., free from other macro-molecules (like proteins, RNA, enzymes, etc.) in order to cut the DNA with restrictor enzymes.
Isolation of genetic material (DNA) is carried out in the following steps:
(a) Since the DNA is enclosed within the membranes, so, in order to release DNA along with other macro-molecules such as proteins, polysaccharides and lipids, bacterial cells/plant or animal tissues are treated with the enzyme lysozyme (bacteria), cellulose (plant cells), chitinase (fungus), respectively.
(b) RNA can be removed by the treatment with ribonuclease, whereas proteins can be removed by the treatment with protease.
(c) Other molecules can be removed by appropriate treatments and ultimately purified DNA will precipitate out, after the addition of chilled ethanol. This can be seen as collection of fine threads in the suspension.
So, the correct answer is “Precipitation of DNA”.
