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Byju's Answer
Standard XII
Biology
Restriction Enzyme
The technique...
Question

The technique involved in the insertion of the desired gene into the DNA of the plasmid vector is known as

A
Dressing
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B
Splicing
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C
Cloning
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D
Drafting
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Solution

The correct option is C Cloning
Cloning is a process in which the DNA segments are joined together and produced in multiple copies in the host cell. Generally, one end of each of the two-segment is connected in order to achieve the new genetic combination. The plasmids are used as vectors to transfer this rDNA into the cell where multiple copies of the DNA fragments can be produced. The method of genetic recombination is often used in the process of cloning.
Thus, the correct answer is option C.

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Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?
Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Bacterial cells receives naked DNA from solution by the process of

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except
Q.

Arrange the following steps involved in gene therapy in a correct order:

i. Isolation of functional or expressible gene to be inserted

ii. Identification of non-functional gene

iii. Insertion of DNA into a vector for delivery into the target cell

iv. Recombinant DNA construction


Q.

Some of the steps involved in Gene Cloning are given below. Arrange them in correct order

(a) insertion of isolated gene to the vector (b) introduction of recombinant vector to the host

(c) isolation of desired gene (d) expression of recombinant gene in the host


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