Lambda Phage

Q.

What is the principle of gene cloning?

Q. Construction of first recombinant DNA was done by using plasmid of

1. Salmonella typhimurium
2. Escherichia coli
3. Bacillus thuringiensis
4. Yeast

Q.

Why are bacteria used in recombinant DNA technology?

Q. Plasmid vector in recombinant DNA technology means

1. any fragment of DNA carrying a desirable gene
2. a virus that transfers genes to the bacteria
3. an extrachromosomal autonomously replicating DNA
4. a gene that does not replicate but readily incorporate into the host DNA

Q. Which among the following are characteristics of an ideal vector?

1. Presence of ori
2. Presence of selectable markers
3. Multiple cloning sites​​​​​​
4. All of these

Q.

Why is genetic recombination important for bacteria?

Q. Viral DNA after being converted from viral RNA by X, incorporates into host genome to undergo replication. What is ‘X’ ?

1. RNA polymerase
2. DNA polymerase
3. Restriction endonuclease
4. Reverse transcriptase

Q. Which one of the following characteristics is generally not preferred for a cloning vector?

1. An origin of replication
2. Multiple restriction sites
3. An antibiotic resistance marker
4. A high copy number

Q. Some foreign DNA fragment is attached to Cla I site of pBR322. This recombinant vector is used to transition Escherichia coli. Then on two different media, one containing ampicillin and the other containing the recombinant vector will

1. grow on both tetracycline and ampicillin containing media
2. not grow on either tetracycline containing or ampicillin containing media
3. grow on tetracycline but not on ampicillin containing medium
4. grow on ampicillin but not on tetracycline containing medium

Q. Which of the following is used in recombinant DNA technique?

1. Cell wall of virus
2. Gene which produces capsid of virus
3. Virus
4. Capsid of virus

Q. What are the properties of ideal cloning vectors?

Q. If the DNA of a virus is labelled with ${}^{32}P$ and the protein of the virus is labelled with ${}^{35}S,$ after transduction which molecules would be present inside the bacterial cells?

1. ${}^{32}P$ only
2. ${}^{35}S$ only
3. Both ${}^{35}Sand{}^{32}P$
4. Neither molecule would be present inside the cell

Q. Why plasmids are preferred as vectors in recombinant DNA technology ?

1. They can easily bind with host DNA
2. They carry antibiotic resistant genes
3. They have the ability to replicate on their own
4. They can shuttle between prokaryotic and eukaryotic cells

Q. A DNA cloning vector should possess

1. ori
2. selectable marker
3. multiple cloning sites
4. all of these

Q. Which of the following are used in gene cloning?

1. Nucleoids
2. Lomasomes
3. Mesosomes
4. Plasmids

Q.

how recombinant DNA is formed?

Q. The technique involved in the insertion of the desired gene into the DNA of the plasmid vector is known as

1. Dressing
2. Splicing
3. Cloning
4. Drafting

Q. A DNA cloning vector should possess

1. ori
2. selectable marker
3. multiple cloning sites
4. all of these

Q. The first person who induced mutations by using X-rays was

1. Muller
2. Mendel
3. Vries
4. Curie

Q. Which of the following is used in recombinant DNA technique?

1. Cell wall of virus
2. Gene which produces capsid of virus
3. Virus
4. Capsid of virus

Q. ___________ is the revolutionary event in biotechnology after cloning.

Q. The flow chart given below represents the process of recombinant DNA technology. Identify A, B, C and D

1. A-Restriction endonuclease, B-Restriction exonuclease, C-DNA ligase, D-Transformation
2. A-Restriction endonuclease, B-Restriction endonuclease, C-DNA ligase, D-Transformation
3. A-Restriction endonuclease, B-Restriction endonuclease, C-Hydrolase, D-Transformation
4. A-Restriction endonuclease, B-Restriction endonuclease, C-Hydrolase, D-Transduction

Q. Mention the uses of cloning vector in biotechnology.

Q. Which of the following is mostly used in genetic engineering experiments?

1. Bacillus subtilis
2. Rhizobium
3. Staphylococcus
4. Escherichia coli

Q. Which of the following is used in recombinant DNA technique?

1. Cell wall of virus
2. Gene which produces capsid of virus
3. Virus
4. Capsid of virus

Q. Match the columns I and II.

	List I		List II
A	Radioactive antibody	1	Substance that can be constructed in the laboratory. Its base sequence can be deduced from the amino acid sequence of the protein it codes for.
B	Artificial gene	2	Substance that can be used to identify colonies of genetically engineered bacteria that make a particular gene product.
C	Amplification	3	The use of entire array of genes of an organism in order to obtain the particular gene product.
D	To produce clone	4	Abnormal enhanced replication of a plasmid in each cell.
E	Shotgun cloning	5	A large population of identical cells.

1. A- 2, B- 3, C- 4, D- 1, E- 5
2. A- 2, B- 1, C- 4, D- 5, E- 3
3. A-1, B- 4, C- 2, D- 5, E- 3
4. A- 2, B- 3, C- 1, D- 4, E- 5

Q.

what is the difference between recombinant and non - recombinant ?

Q. Identify the vector suitable for cloning long DNA fragments :

1. Phage vector
2. Cosmids
3. Yeast plasmid
4. Bacterial plasmid

Q. Which one among the following is just a cloning plasmid and not a expression plasmid?

1. pBAD-18-Cam
2. pBCSK
3. pET
4. pUC19

Q. Which of the following are used in gene cloning?

1. Nucleoids
2. Lomasomes
3. Plasmids
4. Mesosomes