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Question

A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce a 3000 bp and a 2000bp bands when visualized on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A(A1) is present at the 100th base, the order in which the remaining sites (A2,B1 and B2) are present is?

A
3100,5100,8100
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B
8100,3100,5100
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C
5100,3100,8100
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D
8100,5100,3100
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Solution

The correct option is B 3100,5100,8100
When the 10,000 bp plasmid is digested with one restriction enzyme 5000 bp band is obtained. Hence the plasmid has two restriction sites for this enzyme that are located 5000 bp away from each other. The 5000 bp fragments on digestion by the second enzyme results in 2000 and 3000 bp fragments. So the second enzyme has 2 restriction sites. There are 2 variations for the position of the restriction site that is given in the table. Thus, the correct answer is option A.
676773_632929_ans_3ce8ca07da7342878aec0dc56d39143f.PNG

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