MyQuestionIcon

1

You visited us 1 times! Enjoying our articles? Unlock Full Access!

Gene Cloning

Consider the ...

Question

Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

A

1, 3, 2, 4, 5

No worries! We‘ve got your back. Try BYJU‘S free classes today!

B

3, 2, 1, 5, 4

No worries! We‘ve got your back. Try BYJU‘S free classes today!

C

1, 3, 5, 4, 2

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

D

5, 1, 3, 4, 2

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is C $1, 3, 5, 4, 2$
C.1,3,5,4,2
Solution : In a recombinant DNA technology method first we break the donor DNA at specific site by restriction endonuclease. Then we isolate a suitable plasmid from a bacteria and join the DNA with the plasmid to create recombinant DNA. This DNA is inserted into a bacteria or a cell and cultured .
So the correct answer is "1,3,5,4,2"

Suggest Corrections

thumbs-up

0

Similar questions

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

Q. The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

Gene Cloning

BIOLOGY

Watch in App

explore_more_icon

Explore more

Standard X Biology

Join BYJU'S Learning Program

CrossIcon