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Plasmids

Eukaryotic ge...

Question

Eukaryotic genes do not function properly when cloned into a bacterial cell because

A

of high pH present in bacterial cells

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B

of the inability to excise introns and destruction by bacterial restriction enzymes

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C

of inappropriate insertion of genes

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D

eukaryotic genes are monocistronic

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Solution

The correct option is B of the inability to excise introns and destruction by bacterial restriction enzymes
Eukaryotic genes do not function properly when cloned into a bacterial cell because, mRNA in eukaryotes contain coding and non-coding regions called the introns and exons respectively. The introns are non coding regions that are spliced and the exons are adhered together to form the functional mRNA. The mRNA is capped to prevent the action of nucleases. This process cannot take place in a prokaryotic cell because splicing as well as capping process is absent
So, the correct answer is ' the inability to excise introns and destruction by bacterial restriction enzymes'

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Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
All of the following statements is true about restriction enzymes except

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).

Bacterial cells receives naked DNA from solution by the process of

Q. Following steps are involved in cloning a eukaryotic gene in a bacterial plasmid vector.
I. Introduction of cloning vector into cells.
II. Insertion of eukaryotic DNA into the vector.
III. Identification of cell clones that carry the inserted eukaryotic gene.
IV. Isolation of the vector and the eukaryotic gene-source DNA.
V. Cloning of cells (and foreign DNA).
Which of the following statemet is the correct reason for the insertion of eukaryotic DNA into the plasmid vector, the sticky ends formed by digestion of both DNA types with the same restriction enzyme may join in a recombinant molecule?

Q. Assertion: A bacterial cell with restriction enzymes will be easily infected and lysed by bacteriophages.
Reason : Restriction enzymes catalyse synthesis of protective coat around bacterial cell that prevents the attack of bacteriophage.

Q. Assertion :A bacterial cell with no restriction enzymes will be easily infected and lysed by bacteriophages. Reason: Restriction enzymes catalyse synthesis of protective coat around bacterial cell that prevents bacteriophage attack.

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