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Molecular Phylogeny

Given below a...

Question

Given below are the steps of recombinant DNA technology.

(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host

Choose the correct sequence of events.

A

A→C→D→B→E

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B

B→A→D→E→C

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C

C→E→A→D→B

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D

B→D→A→E→C

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Solution

The correct option is D B→D→A→E→C
Recombinant DNA technology is the set of techniques that enable the DNA from different sources to be identified, isolated, and recombined so that new characteristics can be introduced into an organism.

The steps of recombinant DNA technology are:

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Q. The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?

Q. Some of the steps involved in gene cloning are given below. Arrange them in the correct order.
I. Introduction of the identified DNA into the host.
II. Selection of hosts having desired DNA.
III. Identification of DNA with desirable genes.
IV. Transfer of the desired DNA to its progeny.

Some of the steps involved in Gene Cloning are given below. Arrange them in correct order

(a) insertion of isolated gene to the vector (b) introduction of recombinant vector to the host

(c) isolation of desired gene (d) expression of recombinant gene in the host

Q. Recombinant DNA technology involves several steps in specific sequence. Find out the correct sequence.

a. Fragmentation of DNA
b. Culturing the host cells in a medium at large scale
c. Ligation of DNA fragment into a vector
d. Extraction of the desired product
e. Isolation of DNA
f. Isolation of desired DNA fragment
g. Transferring the recombinant DNA into the host

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
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