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Molecular Phylogeny

Given below a...

Question

Given below are the steps of recombinant DNA technology.

(A) Introduction of desired gene into the host
(B) Identification of desired gene
(C) Transfer of desired gene to the progeny
(D) Isolation of desired gene
(E) Maintenance of introduced DNA into the host

Choose the correct sequence of events.

A

A→C→D→B→E

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B

B→A→D→E→C

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C

C→E→A→D→B

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D

B→D→A→E→C

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Solution

The correct option is D B→D→A→E→C
Recombinant DNA technology is the set of techniques that enable the DNA from different sources to be identified, isolated, and recombined so that new characteristics can be introduced into an organism.

The steps of recombinant DNA technology are:

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Q. Some of the steps involved in gene cloning are given below. Arrange them in the correct order.
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II. Selection of hosts having desired DNA.
III. Identification of DNA with desirable genes.
IV. Transfer of the desired DNA to its progeny.

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(c) isolation of desired gene (d) expression of recombinant gene in the host

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a. Fragmentation of DNA
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c. Ligation of DNA fragment into a vector
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B) Isolation of DNA
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