Question

How many selectable markers are present in pBR322?

Answer 1

Selectable marker:

1. The selectable marker could be a gene present within the vector that, when introduced into the host or cells in culture, results in a trait suitable for artificial selection.
2. Selectable markers for antibiotic resistance are commonly used.
3. These aid in the artificial selection process.

Selectable markers in pBR322:

1. The cloning vector pBR322 contains selectable markers for ampicillin and tetracycline resistance genes.
2. They play a supportive role in the differentiation of transformed cells from non-transformed cells.
3. They also aid in distinguishing between recombinant and non-recombinant cells.