Question

In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

• A. Charge only
• B. Charge to size ratio
• C. Size only
• D. All of the above

Answer 1

The correct option is C Size only

Agarose gel electrophoresis:

Agarose gel electrophoresis is the process of separation of DNA fragments in an electric field based on their size.

● The DNA fragments and size markers are loaded onto the gel well and current is passed through the gel.

● The DNA being negatively charged, moves towards the positive electrode during gel electrophoresis.

● The smaller the size of the molecule, the faster and farther it travels. This is how the fragments of varying sizes are separated.

Final answer:

b. Size only