Question

In DNA fingerprinting, DNA hybridization with the help of a specific DNA probe is done through

• A. Northern blotting
  
• B. Western blotting
  
• C. Southern blotting
  
• D. Eastern blotting
  

Answer 1

The correct option is C Southern blotting


Southern blotting

Southern blotting is an analytical tool used for the specific identification of desired DNA from thousands of DNA molecules.

• The DNA bands formed by gel electrophoresis are transferred to the nitrocellulose membrane and detected using hybridization with a radiolabelled VNTR probe.
• Thus, Southern blotting is involved in DNA fingerprinting.

Final answer: (c) Southern blotting