In the diagram of pBR 322, which of the following is true about the components?
A
rop - reduced osmotic pressure
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B
EcoRI, BamHI - Selectable markers
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C
ampr - Antibiotic resistance gene
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D
Ori - Original restriction enzyme
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Solution
The correct option is C ampr - Antibiotic resistance gene The following diagram shows the basis components of E. coli cloning vector pBR322:
The functions of a few components in a plasmid - ampr - antibiotic resistance gene. They enable the bacteria to survive the effects of the antibiotic ampicillin and serves as a characteristic to recongnise transformed cells. ori - Origin of replication. rop - gene coding for the Rop protein, which promotes conversion of the unstable RNA I – RNA II complex to a stable complex. EcoRI and BamHI - restriction sites