1
Question
Source of Taq polymerase used in PCR is a
Source of Taq polymerase used in PCR is a
Open in App
Solution
The correct option is D Thermophilic bacterium
Taq polymerase is a thermostable enzyme which is used to catalyse the addition of dNTPs to the primer for the extension of the DNA strand. This is carried out at 72∘C.
The temperature of subsequent cycles of PCR, especially the denaturation step, occurs at 94−95∘C. Hence, the thermostable nature of DNA polymerase is a must for PCR.
Therefore this enzyme is isolated from a bacteria, Thermus aquaticus that lives at high temperatures.
Thermophilic bacteria are able to live at relatively high temperatures, ranging between 41−122∘C. Examples include Thermus aquaticus.
Thermophilic fungi are able to live between a temperature range of 45−55∘C. They are used in fermentation. Examples include Myceliophthora thermophila.
Mesophilic fungi are able to grow at temperatures in the range of 5−35∘C. It is used in composting. Examples include Mucor mucedo.
Halophilic bacteria are able to live in environments such as salt lakes, saline soils, and salted food products. Examples include Salinibacter, Halomonas etc.
Hence the correct option is
c. Thermophilic bacterium
Taq polymerase is a thermostable enzyme which is used to catalyse the addition of dNTPs to the primer for the extension of the DNA strand. This is carried out at 72∘C.
The temperature of subsequent cycles of PCR, especially the denaturation step, occurs at 94−95∘C. Hence, the thermostable nature of DNA polymerase is a must for PCR.
Therefore this enzyme is isolated from a bacteria, Thermus aquaticus that lives at high temperatures.
Thermophilic bacteria are able to live at relatively high temperatures, ranging between 41−122∘C. Examples include Thermus aquaticus.
Thermophilic fungi are able to live between a temperature range of 45−55∘C. They are used in fermentation. Examples include Myceliophthora thermophila.
Mesophilic fungi are able to grow at temperatures in the range of 5−35∘C. It is used in composting. Examples include Mucor mucedo.
Halophilic bacteria are able to live in environments such as salt lakes, saline soils, and salted food products. Examples include Salinibacter, Halomonas etc.
Hence the correct option is
c. Thermophilic bacterium
Suggest Corrections
0
Similar questions
View More
Join BYJU'S Learning Program
Explore more
Join BYJU'S Learning Program
