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Question
The thermostable enzymes 'Taq' and 'Pfu' isolated from thermophilic bacteria are :
The thermostable enzymes 'Taq' and 'Pfu' isolated from thermophilic bacteria are :
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Solution
The correct option is C DNA polymerases
DNA polymerase
- DNA polymerases are the enzymes that can add deoxyribonucleotides to an already existing DNA strand or primer.
- Hence DNA polymerases are used for in vitro DNA synthesis.
- The thermostable property of thermostable DNA polymerases makes it possible for them to remain active during the high temperature conditions of PCR, especially during the denaturation of double stranded DNA.
- Examples of such thermostable DNA polymerases include ‘Taq and Pfu’ isolated from thermophilic bacteria.
DNA polymerase
- DNA polymerases are the enzymes that can add deoxyribonucleotides to an already existing DNA strand or primer.
- Hence DNA polymerases are used for in vitro DNA synthesis.
- The thermostable property of thermostable DNA polymerases makes it possible for them to remain active during the high temperature conditions of PCR, especially during the denaturation of double stranded DNA.
- Examples of such thermostable DNA polymerases include ‘Taq and Pfu’ isolated from thermophilic bacteria.
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