Question

What are the restriction sites in pBR322?

Answer 1

pBR322:

1. pBR322 is the plasmid of Bolivar and Rodrigues.
2. pBR322 serves as a vector for creating recombinant DNA in E.coli.
3. pBR322 comprises an origin of replication, two antibiotic-resistant genes (for tetracyclin and ampicillin), and five restriction sites.

Restriction sites:

1. Restriction sites are specific DNA sequences that allow a specific restriction enzyme to cleave that region.
2. The gene of interest can then be inserted here.
3. Thus, restriction sites are simply areas of inserting our genes of interest after slicing them with the restriction enzyme whose DNA sequence is complementary to it.

Restriction sites in pBR322 are:

1. EcoRI
2. HindIII
3. BamHI
4. SalI
5. PstI