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Question

What is ligase in DNA replication?


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Solution

DNA Replication:

  1. During replication, DNA ligase binds the Okazaki fragments that were synthesised in a random order.
  2. DNA ligase is an enzyme that repairs breaks or abnormalities in the double-stranded DNA backbone.
  3. It has three main functions: it closes DNA repairs, joins Okazaki fragments, and seals recombination fragments (small DNA fragments formed during the replication of double-stranded DNA).
  4. The end of a "donor" nucleotide and the end of a "acceptor" nucleotide create a link, which is how DNA ligase works.
  5. DNA ligase is a key component in genetic recombination research, including cloning, because it assists with DNA repair and replication.


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