Question

What is ligase in DNA replication?

Answer 1

DNA Replication:

1. During replication, DNA ligase binds the Okazaki fragments that were synthesised in a random order.
2. DNA ligase is an enzyme that repairs breaks or abnormalities in the double-stranded DNA backbone.
3. It has three main functions: it closes DNA repairs, joins Okazaki fragments, and seals recombination fragments (small DNA fragments formed during the replication of double-stranded DNA).
4. The end of a "donor" nucleotide and the end of a "acceptor" nucleotide create a link, which is how DNA ligase works.
5. DNA ligase is a key component in genetic recombination research, including cloning, because it assists with DNA repair and replication.