Molisch’s test is a chemical test that is used to check for the presence of carbohydrates in a given sample.
This test is named after Austrian botanist Hans Molisch.
Molisch’s test involves the addition of Molisch’s reagent {a solution of α-naphthol in ethanol } to the sample and the subsequent addition of a few drops of concentrated (sulphuric acid ) to the mixture.
Tetrose and triose sugars don’t give Molisch’s test.
The principle of Molisch's test is the dehydration of sulphuric acid into furfural.
Positive Molisch's Test
The formation of a purplish-red ring at the point of contact between sulphuric acid and the analyte + Molisch’s reagent mixture confirms the presence of carbohydrates in the sample.