Gram-positive bacteria appear bluish-purple colored under the microscope when treated with Gram's stain.
They have a very thick layer of peptidoglycan and hence can retain crystal violet stain.
Some of the examples of gram-positive bacteria are Actinomyces, Clostridium, Mycobacterium, Streptococci, Staphylococci, and Nocardia.
In Gram-positive bacteria, the reason for this negative charge is due to the presence of teichoic acids bound to the peptidoglycan or to the underlying plasma membrane.
The teichoic acid is having a negative charge due to the presence of phosphates in its structure.