Question

What 'X'and 'Y' indicated in given diagram ?

• A. X – Plasmid, Y– Foreign DNA
• B. X – Foreign DNA Y – Sticky ends
• C. X – Sticky ends, Y – foreign DNA
• D. X – Plasmid and Y – Sticky ends

Answer 1

The correct option is D X – Plasmid and Y – Sticky ends

Restriction endonucleases are used in genetic engineering to form 'recombinant' molecules of DNA, which are composed of DNA from different sources/genomes.

Sticky ends are helpful in cloning because they hold two pieces of DNA together so they can be linked by DNA ligase. Sticky ends are more useful in molecular cloning because they ensure that the human DNA fragment is inserted into the plasmid in the right direction. The ligation process, or fusing of DNA fragments, requires less DNA when the DNA have sticky ends.

Plasmids are extra-chromosomal DNA molecules that replicate independent of chromosomal DNA. It has its own origin of replication. It carries many genes which benefits bacteria for survival. It contains antibiotic resistance genes.

So, the correct answer is "option D"