Question

Which is not a key step in the process of DNA molecular cloning?

• A. Heat shock at ${42}^{o}C$ for 90 seconds.
• B. Make DNA competent to take up plasmid with use of calcium chloride
• C. Seal plasmid carrying targeted genes into host cells genome using ligase.
• D. Introduce genes for cloning into a plasmid vector using restriction enzymes.

Answer 1

The correct option is A Heat shock at ${42}^{o}C$ for 90 seconds.

A- The steps involved in the process of DNA molecular cloning are – selected DNA fragment is inserted into a plasmid utilizing restriction enzymes and DNA ligase enzymes. The produced molecule of recombinant DNA is then introduced into a selected and grown up bacteria which reproduce by producing an exact copy of the plasmid and transferring it to their progeny.

So, the correct answer is 'Heat shock at 42oC42oC for 90 seconds'