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Hydrolases

Why Hind II i...

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Why Hind II is the first restriction enzyme not Hind I?

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Q. The DNA restriction site recognized by the enzyme Hind

I I I

\frac{A A G C T T}{T T C G A A}

Which of the following sequence contained in a double stranded DNA is cut by Hind

I I I

?

Q. Which among the following restriction endonucleases will produce blunt ends?
I. BamH I
II. Hind II
III. EcoR I
IV. Hind III
V. Sal I

Q. Restriction endonuclease Hind II always cuts DNA molecule at a particular point by recognising a specific sequence of

Q. Read the following statements and select the correct ones.
(i) Same kind of sticky ends are produced when a DNA is cut by different restriction enzymes.
(ii) Exonucleases make cuts at specific positions within the DNA.
(iii) Hind II was the first restriction endonuclease to be isolated.
(iv) A bacteriophage has the ability to replicate within bacterial cells by integrating its DNA with bacterial DNA.
(v) Presence of more than one recognition site for the same restriction enzyme within the vector complicates gene cloning.

Q. In nomenclature of REN (restriction endo - nuclease)

H i n d

I I I

, III stands for

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