The foreign DNA is inserted into a plasmid by cutting the DNA and plasmid by the same restriction enzyme and then ligating the foreign DNA into... View Article
Yes, pBR322 is an artificial cloning vector created in the laboratory of Herbert Boyer. BR stands for the names of the scientists, Bolivar and... View Article
pBR322 is a good cloning vector because it contains two antibiotic resistance genes that act as a selectable marker and recognition sites for... View Article
DNA vectors are used for cloning. They are used for transferring foreign DNA into another cell. Further reading: Plasmid What Is EcoR1?... View Article
Yeast artificial chromosome (YAC) is used for cloning larger DNA fragments. It can be used to clone up to 3000 kb of DNA fragment. Further... View Article
Yes, plasmids are small, circular and double-stranded DNA that replicate autonomously. They are extrachromosomal DNA molecules. Further reading:... View Article
Plasmids are used as cloning vectors in genetic engineering because they can replicate independently and can transfer the desired gene to the... View Article
Plasmids are self-replicating extrachromosomal DNA present in bacteria. They contain cloning sites where foreign DNA can be inserted, a... View Article
The main characteristics of a cloning vector are: Origin of replication for replication within the host cell. Selectable markers such as... View Article
Both plasmid and foreign DNA are cut by the same restriction enzyme and then the foreign DNA is ligated into the complementary site of the... View Article
pBR322 is a plasmid. It contains various restriction sites in the antibiotic resistance genes, tetracycline and ampicillin. It is used for... View Article
The main types of cloning vectors are plasmids, bacteriophages, cosmids, yeast artificial chromosomes (YACs), bacterial artificial chromosomes... View Article
Cloning vectors are a small genome, where foreign DNA can be inserted and delivered to the host cell that can be used for cloning purposes. E.g.... View Article
Cloning vectors are used to insert foreign DNA into a host cell and make multiple copies of the inserted DNA. Further reading: Plasmid What... View Article
Sticky ends or overhanging single-stranded stretches cut facilitates ligation by DNA ligase due to the formation of hydrogen bonds between... View Article
