Blunt End Ligation
Endonucleases and exonucleases are the restriction enzymes used in molecular techniques. They cut the desired DNA portion. Usually, a straight cut creates blunt ends or non-overhanging ends. These ends can be joined using a DNA ligase enzyme. The joining of two blunt ends is called blunt end ligation. This does not need matching or complementary ends for ligation.
Sticky End Ligation
Likewise, the restriction enzymes creating a staggered cut leads to two sticky ends or overhanging ends. The ligation between two overhanging ends with matching or complementary bases is called sticky end ligation. This sticky end ligation is more efficient than blunt end ligation. Thus, it is the most desired process in cloning techniques.
Difference between Blunt and Sticky End Ligation
Blunt End Ligation |
Sticky End Ligation |
|
This involves the ligation of two blunt ends. |
This involves the ligation of two sticky ends. |
|
Usually, a straight cut by restriction enzymes creates non-overhanging or blunt ends. |
Usually, a staggered cut by restriction enzymes create overhangs or sticky ends. |
|
The restriction enzymes used are EcoRV, SmaⅠ. |
The restriction enzymes used are EcoR1, HindⅢ. |
|
No complementary or matching strands are needed for ligation. |
Matching or complementary strands are needed for ligation. |
|
Less efficient |
More efficient. |
|
These ends are not favoured in biotechnology. |
These ends are most desired in biotechnology. |
Also Read: What is EcoRⅠ?
Frequently Asked Questions
What is DNA ligation?
Extended Reading: DNA Ligases
What are restriction enzymes?
The restriction enzymes include a group of enzymes that cleave the DNA molecules. They usually cleave the sugar-phosphate backbone. The endonucleases are restriction enzymes that cleave DNA at specific positions. Whereas, exonucleases are another group of restriction enzymes that removes nucleotides from the DNA ends.
Also see: Biotechnology and its Principles.
Keep exploring BYJU’S Biology for more exciting topics.
Comments