Identification of Stages of Gamete Development, i.e., T.S. of Testes and T.S. of Ovary Through Permanent Slides (from Grasshopper, Mice)

Aim

To identify the stages of gamete development, i.e., T.S. of testes and ovary through permanent slides from mice.

Materials Required

• Permanent slides of T.S. of testes.
• Permanent slides of T.S. of the ovary.

Procedure

T.S. of Testes of Mice

• The testes comprise several seminiferous tubules embedded in the interstitial tissues.
• Thick fibrous tissues called tunica albuginea cover the testes.
• It comprises different types of cells from the outside to the lunar in the manner given below:

Spermatogonia → Spermatocytes → Spermatids → Spermatozoa (sperms)

• Sertoli cells are located between the germinal cells.
• The Leydig cells that produce testosterone are present in the interstitial tissues.

T.S. of Ovary of Mice

• An ovary is a germinal epithelium bounded by a solid structure covered by a thick layer of fibrous tissue known as tunica albuginea.
• It consists of an inner medulla and an outer cortex.
• The medulla comprises several round or oval bodies known as ovarian follicles.
• Follicle development takes place in the following stages:

1°follicle → 2°follicle → 3°follicle → Graffian follicle → Corpus luteum

• Cortex comprises corpus luteum along with mature follicles.

Precautions

• The microscope should be handled with care.
• Adjust the lens such that the focus is better.

Learn more in detail about the stages of gamete development, other related topics and experiments at BYJU’S Biology.