Aim

To identify the stages of gamete development, i.e., T.S. of testes and ovary through permanent slides from mice.

Materials Required

• Permanent slides of T.S. of testes.
• Permanent slides of T.S. of the ovary.

Procedure

T.S. of Testes of Mice

• The testes comprise several seminiferous tubules embedded in the interstitial tissues.
• Thick fibrous tissues called tunica albuginea cover the testes.
• It comprises different types of cells from the outside to the lunar in the manner given below:

Spermatogonia → Spermatocytes → Spermatids → Spermatozoa (sperms)

• Sertoli cells are located between the germinal cells.
• The Leydig cells that produce testosterone are present in the interstitial tissues.

T.S. of Ovary of Mice

• An ovary is a germinal epithelium bounded by a solid structure covered by a thick layer of fibrous tissue known as tunica albuginea.
• It consists of an inner medulla and an outer cortex.
• The medulla comprises several round or oval bodies known as ovarian follicles.
• Follicle development takes place in the following stages:

1°follicle → 2°follicle → 3°follicle → Graffian follicle → Corpus luteum

• Cortex comprises corpus luteum along with mature follicles.

Precautions

• The microscope should be handled with care.
• Adjust the lens such that the focus is better.

Learn more in detail about the stages of gamete development, other related topics and experiments at BYJU’S Biology.