Microbes are tiny structures that are not visible to the naked eye, they are microscopic in nature. Some microbes can be extremely beneficial while some others can be fatal enough. These miniature creatures accelerate several huge biological processes constantly occurring in nature without human intervention. Some microorganisms are responsible for running industries such as the baking industry(yeast), dairy industry(lactobacillus) etc. Let us have a detailed look at the role of microbes in everyday life and its significance in stimulating chemical reactions and understand how these processes bring about a balance in the ecosystem.
Very Short Answer Type Questions
Q.1. Why are there big holes in ‘Swiss cheese’?
A.1. It is because of the bacterium named Propionibacterium shermanii that releases large amounts of carbon dioxide.
Q.2. What is a fermentor?
A.2. To cater to large scale production of biotechnological products such as beverages and antibiotics in industries, microbes are grown in large vessels known as bioreactors or fermentors.
Q.3. Give an example of a microbe that is used in statin production. How do they lower blood cholesterol level?
A.3. Statins are produced by yeasts named Monascuspurpureus and are bioactive molecules. They have been commercialized as agents that aid in lowering the blood cholesterol levels. It competitively inhibits the action of the enzymes that synthesize cholesterol.
Q.4. Why is secondary wastewater treatment referred to as biological approach?
A.4. It is because microbes are involved in the breakdown of organic matter in this stage of wastewater management. Primary effluents are passed into aeration tanks where organic matter contained in it is absorbed by aerobic microbes which are later digested by anaerobic fungi and bacteria in sludge digesters.
Q.5. State the use of Nuclear Polyhedrosis Virus.
A.5. They are excellent candidates for narrow-spectrum, species-specific insecticidal applications which have shown to have no negative impact on mammals, plants, birds, fish or even non-target insects that is desirable when beneficial insects are being conserved to promote the Integrated Pest Management(IPM) programme.
Q.6. What is the significance of antibiotics in medicine?
A.6. They are chemical substances, produced by a few microbes that have the potential to kill or retard the growth of disease-causing microbes. They have greatly enhanced human capacity to treat deadly diseases such as plague, diphtheria, leprosy, whooping cough etc.
Q.7. Why do we distil to produce a few alcoholic drinks?
A.7. It is because distillation raises the alcohol content in a few alcoholic drinks.
Q.8. Name the common trait shared between Clostridium butylicum, Lactobacillus and Aspergillus niger.
A.8. As part of their metabolic activities, they produce organic acids which can be used for their industrial and commercial production. Clostridium butylicum – butyric acid, Lactobacillus-lactic acid and Aspergillus niger – citric acid.
Q.9. What if human intestine nourished microbial flora correctly equivalent to the one found in the rumen of cattle?
A.9. We would be able to digest cellulose in our food as microbes(methanogens) present in the cattle’s rumen can digest cellulose since they contain the enzyme cellulase.
Q.10. Name two microbes beneficial in biotechnology.
A.10. Escherichia coli and Bacillus thuringiensis
Q.11. Name the organism responsible for Eco RI, restriction endonuclease?
A.11. Escherichia coli strain RY 13
Q.12. List any crop that is genetically modified.
A.12. Bt cotton developed by inserting a gene from Bacillusthuringiensis is genetically modified to resist attack from insect pests.
Q.13. Why are blue-green algae not as popular as biofertilizers?
A.13. It is due to several environmental constraints such as infrastructural, technological, financial, quality marketing, unawareness etc.
Q.14. Roquefort cheese is obtained from which of the species of Penicillin?
A.14. Fungi Penicilliumroqueforti
Q.15. Which states are involved in the Ganga action plan?
A.15. Jharkhand, UP, Bihar, West Bengal.
Q.16. List two industrially significant enzymes.
(i) Proteases and pectinases are used in making commercial fruit and vegetable juices are clarifying agents
(ii)They are helpful in removing oily stains from the laundry as upases are used in detergent formulations.
Q.17. List an immune immunosupressive agent.
A.17. The fungus Trichodermapolysporum produces Cyclosporin-A which is used as an immunosuppressive agent in organ transplant patients.
Q.18. Name a rod-shaped virus.
A.18. Tobacco mosaic virus
Q.19. Name the class of bacteria found both in the sludge of sewage treatment and in the rumen of cattle.
Short Answer Type Questions
Q.1. What is the significance of floes in the biological treatment of wastewater?
A.1. They are portions of bacteria related to fungal filaments which form mesh-like structures. It helps in digestion of organic matter, eliminate pathogens and liberate nutrients in the sewage effluents.
Q.2. What is the role of the bacteria Bacillus thuringiensis in regulating caterpillars of insect pests?
A.2. It produces an endotoxin which upon ingestion and liberation into the gut of the larvae of insect pest, destructs the gut lining of the insect hence killing it.
Q.3. The fungi mycorrhizal benefit the plant in which it harbours, How?
A.3. It absorbs phosphorous from the soil and transfers to the host cells. They also provide resistance against root pathogens to the host plants. It promotes the overall development and growth of the plant and shows tolerance to drought and salinity. Thus fungal hyphae aids in producing organic food and also procure nourishment and shelter from higher plants.
Q.4. How are cyanobacteria used in the fields of paddy?
A.4. They fix nitrogen from the atmosphere and supplement organic matter to increase the fertility of the soil. Examples – Nostoc, Anabaena, Oscillatoria.
Q.5. Write about the discovery of Penicillin.
A.5. It was the first antibiotic to be discovered and is an accidental discovery. Alexander Fleming noted a mould of Penicillium growing in unlaundered culture plates of Staphylococcus. The mould hindered the growth of Staphylococcus. The antibiotic was later isolated from the fungus to be named after the mould Penicilliumnotatum.
Q.6. Who was credited for demonstrating the significance of Penicillin as an antibiotic?
A.6. Howard Florey and Ernst Chain.
Q.7. How is the restoration of good health in humans brought about by bioactive molecules of fungal origin?
A.7. Cyclosporin-A is utilized as an immunosuppressant in organ transplant patients which is generated by the fungus Trichodermapolysporum. The yeast Monococcuspurpureus produce Statins which are commercially used as agents that lower blood cholesterol levels.
Q.8. State the significance of enzymes in detergents used for washing clothes. Are they produced from unique microbes?
A.8. Lipases are used in formulations of detergent that result in the breakdown of oil hence aids in the removal of grease and stains of oil from clothes. They are obtained from Geotrichumcandidum and Candida lipolytica.
Q.9. Write the chemical nature of biogas. Name one organism which produces biogas.
Chemical nature of biogas – CH4 , CO2 , H2.
A variety of methanogen – Methanobacteria is involved in biogas production.
Q.10. What is the role of microbes in reducing environmental degeneration caused by chemicals?
A.10. Microbes are being used as pesticides and fertilizers to reduce environmental degradation and are called as biopesticides and biofertilizers respectively. As biofertilizers, microbes enrich soil nutrients by fixing atmospheric nitrogen in the soil, examples – Azospirillum, Azotobacter, Rhizobium etc. As biopesticides(Bacillus thuringiensis) they check the growth of insect pests. A fungal species – Trichoderma is effectively used as a biocontrol agent for many plant pathogens. Baculovirus in genus nucleopolyhedrovirus is used as a biological control agent for narrow spectrum, species-specific insecticidal applications.
Q.11. Describe broad-spectrum antibiotic. Give an example.
A.11. It hinders the maturity of both gram-negative and gram-positive bacteria. Example – Jetracyclines, Phenicols, Fluoroquinolones.
Q.12. What is another name used to address the viruses parasitizing bacteria?
A.12. Bacteriophages. They are viruses which do not eat bacteria. They infect and replicate within the bacteria.
Q.13. Name the bacteria which is used as a clot buster. Mention about its mode of action.
A.13. Bacterium Streptococcus secretes Streptokinase which is used as a clot buster. It has a fibrinolytic action which breaks down the clots that are formed in the blood vessels of patients that undergo myocardial infarction which prevents heart attack in such patients that can otherwise occur because of occlusion by clots.
Q.14. Giving two examples describe biofertilizers.
A.14. Biofertilizers supplement the nutrient quality of the soil. Their main source is fungi, bacteria and cyan bacteria. Fizotobacter, Rhizobium can fix atmospheric nitrogen in the soil. Blue-green algae like Nostoc, Anabaena add organic matter to the soil and increase soil fertility.
Long Answer Type Questions
Q.1. For the execution of massive volumes of waste waters rich in organic matter, why is aerobic degradation more important than anaerobic degradation?
A.1. It is because facultative and aerobic microbes that occur naturally such as protozoa, fungi, bacteria etc residing in the wastewater can rapidly oxidize soluble organic and nitrogenous compounds. The process if further accelerated due to mechanical addition of oxygen hence eliminating most of the pathogenic content of the effluent.
Q.2. Discuss the main ideologies crucial in the biological control of diseases and pests.
A.2. The fundamental idea is it is natural and eco-friendly. It involves the utilization of entities to monitor the community of pests and pathogens in an ecosystem. An example is Trichoderma, it is an antagonist which acts against certain soil-borne plant pathogens. Likewise, Penicillin hinders the growth of Staphylococcus and hence has been used in the penicillin production to check several bacterial pathogens. Baculovirus in genus Nucleopolyhedrovirus is used as a biological control agent for the species-specific narrow-spectrum insecticidal application. Bacillus thuringiensis is used to control insects as it acts as a biopesticide. The complete biological regulation of pest is recognised to be much more advantageous in an ecologically sensitive area.
a) What is the consequence of discharging larger volumes of sewage that is untreated into a river?
b) What is the significance of anaerobic sludge digestion in sewage treatment?
A.3. a) If water is untreated and dispensed directly into rivers it will lead to adverse water pollution with pathogenic bacteria, protozoa and organic matter. If this water is consumed, it would lead to outbreaks of water-borne diseases. b) Anaerobic bacteria digest the aerobic bacteria and the fungi that are present in the sludge and the residual organic matter in anaerobic sludge digestion. During this digestion, a mixture of gases is produced such as hydrogen sulphide, methane and carbon dioxide. These biogases can be utilized as a source of energy as it is inflammable.
Q.4. Describe the kind of food that would have lactic acid bacteria. Mention their useful applications.
A.4. Lactic Acid Bacteria(LAB) of the Lactobacillus species is most commonly found in food items such as curd and yoghurt. During the formation of curd, a small amount of curd is added to milk which acts as an initiator. Microbes present in the starter rapidly multiply at suitable temperatures, thereby causing the conversion of milk into curd. The LAB releases acids during the growth curdle and digest the milk protein partially which thereby facilitates the digestibility of the milk protein. Few of its applications are:
Enhances the nutritional quality of milk by increasing vitamin B-12 content
Regulates disease-checking microbes in the stomach
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