Aim

To observe pollen germination on stigma through a permanent slide.

Materials Required

1. Microscope
2. Permanent slide

Procedure

The permanent slide is placed under a microscope and the pollen germination is observed.

Theory

Pollination refers to the transfer of pollen grains from the anther of a flower to the stigma of the same or different flower through biotic or abiotic means. The complete process of pollination is as listed below:

• Once the pollen grains are deposited on the stigma, it starts to germinate with the absorption of nutrients and water.
• A small pollen tube is produced through the style to the ovary.
• The tube cell moves out of the pollen grain and through one of the germ pores forms a pollen tube.
• The nucleus of the tube moves down to the tip of the pollen tube.
• The generative cells also pass into it and soon divide to form two male gametes.
• During double fertilization, one of the two sperms fuses with the egg cell of the ovule. This helps in embryo development.
• The other cell combines with another subsidiary nuclei of the ovule that helps in the formation of the endosperm.
• The growing ovule is transformed into a seed.

Also Read: Types of Pollination

Learn more in detail about the pollen grain germination and other related topics and experiments at BYJU’S Biology.