Monohybrid Cross - Inheritance Of One Gene

The mystery of genetics was unlocked during the mid-nineteenth century by Gregor Mendel. He conducted an experiment on pea plants. By cultivating the pea plants and observing the pattern of inheritance in stages of generation.

Mendel’s Experiment

Mendel is the father of genetics. The Mendel’s laws are Law of Independent Assortment, Law of Dominance, and Law of Segregation. These laws came into existence by experiments on pea plants with a variety of traits.

Mendel investigated the pairs of pea plants with one contrasting trait. Mendel studied on the following seven characters with contrasting traits:

  • Flower color: Violet/white
  • Flower position: Axial/terminal
  • Pod color: Green/yellow
  • Pod shape: Inflated/constricted
  • Seed color: Yellow/green
  • Seed shape: Round/wrinkled
  • Stem height: Tall/dwarf

Monohybrid Cross

Monohybrid Cross – Example And The Ratio

For monohybrid cross, Mendel began with a pair of pea plants with two contrasting traits i.e., one tall and another dwarf. The cross-pollination of tall and dwarf plants resulted in tall plants. All the hybrid plants were tall. He called this as a first hybrid generation (F1) and offspring were called Filial1 or F1 progeny. He conducted an experiment with all seven contrasting pairs. He observed that the entire Fprogeny showed one pattern in their behaviour i.e., they resembled either one of the parents. Another parent character was completely absent.

Monohybrid cross generation

He continued his experiment with self-pollination of F1 progeny plants. Surprisingly, he observed that one out of four plants were dwarf while other three were tall. The tall and the short plants were in the ratio of 3:1. He also noted that no progeny was in intermediate height i.e., no blending. The result was same for other traits of plants too. And he called them second hybrid generation and offspring were called Filial2 or F2 progeny.

Monohybrid cross generation

Mendel observed that traits were absent in F1 generation had reappeared in F2 generation. He called such suppressed traits as recessive traits and expressed traits as dominant traits. He also concluded that some ‘factors’ are inherited by offspring from their parent over successive generations.

Later, these ‘factors’ were called genes. Genes are responsible for the inheritance of traits from one generation to another. Genes consist of a pair of alleles which code for different traits. If a pair of alleles is the same i.e., TT or tt, such alleles are called homozygous pair while those that are different or non-identical (e.g. Tt) are called heterozygous pair.

The cross between two monohybrid traits (TT and tt) is called a monohybrid cross. Monohybrid cross is responsible for the inheritance of one gene. It can be easily shown through a Punnett Square.

How To Carry Out A Monohybrid Cross?

The ratios of the phenotype and the genotype that are estimated are only probabilities. Listed below are steps that can be used to calculate a monohybrid cross:

  1. Indicate the alleles using characters – recessive alleles can be indicated by lower case letters while dominant alleles can be indicated by upper case letters
  2. Note down both the phenotype and the genotype of the parents or the parental generation that are being crossed
  3. Jot down the genotype of the gametes from the parental generation – As a result of meiotic division, the gametes will be haploid
  4. Tabulate a Punnett square to chalk out the probable combinations of the gametes – Any combination is possible is the process of fertilization is random
  5. The phenotype and the genotype ratios of the prospective offspring can be written. The outcome hence obtained is known as the F1 generation. The F2, F3 etc generations form the subsequent generations.

Dihybrid Cross

There are two types of breeding processes to know the mechanism of genes and examine the inheritance of traits from parents and grandparents, one is monohybrid cross and the other is dihybrid cross. A dihybrid cross occurs when the F1 generation offsprings differ in two traits. It is a cross between two entities that are heterozygous for two different traits.

Mendel carried out the following experiment for dihybrid cross:

  • For crossing, he took a pair of contradicting characteristics or traits
  • Mendel crossed round-yellow seed and wrinkled-green seed
  • In the F1 generation, the outcome was seeds that were round and yellow
  • The F1 generation indicated that the round and yellow traits are dominant while the green colour and the wrinkled shape were recessive traits.
  • Self-pollination of F1 progeny resulted in four varying combinations of seeds in the subsequent generation, the F2 generation.
  • The outcome and the dihybrid cross ratio were – round-yellow, wrinkled-yellow, wrinkled-green, round-green and the ratio was – 9:3:3:1.


A testcross is a cross that involves mating with a genotype that is unknown with a known genotype, a homozygous recessive genotype.

A homozygous recessive genotype is crossed because of the following:

  • In the presence of dominant alleles, the effects of recessive alleles are always masked
  • Thereby, the phenotype of the offspring exhibits the genotype of the unknown parent.

Purpose of test cross: They are used to determine if a dominant phenotype is heterozygous or homozygous

Learn more about monohybrid cross and other related biology concepts at BYJU’S.

Read Also:


Practise This Question

__________ were the first living organisms on Earth