Blood Group Test

Blood Group Test

Our blood cell includes red blood cells, white blood cells, and platelets. It plays a significant role in transportation, protection and in regulation. Blood groups are mainly determined by the presence or absence of antigens and antibodies on the surface of our red blood cells or erythrocytes. Both antigens and antibodies are the protein molecules which functions as body’s natural defense. This is the reason blood transfusion from the wrong blood group can be life-threatening.

The four major blood groups are: A, B, AB and O. The blood grouping system was discovered by Austrian biologist and immunologist Karl Landsteiner in the year 1901.

Aim

The main purpose of conducting this experiment is to understand the basic concept of ABO blood group system.

Materials Required

Toothpicks

Blood sample

Alcohol Swabs

Blood Lancet

Clean glass slide

Sterile cotton balls

Biohazard disposal container

Monoclonal Antibodies (Anti-A, B, and D)

Procedure

Take a clean glass slide and draw three circles on it.

Unpack the Monoclonal Antibodies (MAB) kit. In the first circle add Anti-A, to the second circle add Anti-B and to the third circle add Anti-D with the help of a dropper.

Keep the slide under disturb.

Now wipe the ring finger with the alcohol swabs and rub gently near the fingertip, where the blood sample will be collected.

Prick the ring fingertip with the Lancet and a wipe off the first drop of the blood.

As blood starts oozing out, allow it to fall on the three circles of the glass slide by gently pressing the fingertip.

Apply pressure on the site where it was pricked and to stop blood flow. Use the cotton ball if required.

Mix the blood sample gently with the help of a toothpick and wait for a while to observe the result.

Conclusion

Here is the chart which predicts the different types of blood groups along with its Rh factor.

Precautions.

Discard the alcohol swabs, lancet, cotton balls and toothpick after their use. Drop all the materials, including the glass slide into the Biohazard disposal container after observing the result.


Practise This Question

During dark reaction for fixation of carbon, the three carbon atoms of each molecule of 3 phosphoglyceric acid (PGA) are derived from