Biotechnology and Its Principles

Biotechnology is the use of technology for modifying or manipulating a biological system. Biotechnology utilises a living system for making different products.

Biotechnology is widely used in several fields such as agriculture, medicine, genetics and production of various commercial products such as alcohol, wine, chemicals, etc. People have been using biotechnology for ages for brewing and baking purpose.

Also Refer: Role of Biotechnology in human welfare



What is Biotechnology

Biotechnology is an amalgamation of biology and technology for our betterment and sustainable development. Modern biotechnology includes genetic engineering, bioinformatics and bioprocess engineering.

Genetic engineering is used to alter genetic composition of an organism. It is widely used to insert a gene of choice like pest-resistant or antibiotic-resistant genes, etc. to a host by using a cloning vector. It integrates the desirable gene in the host genome and transformation can be seen in the phenotype of the host.

In Bioprocess engineering, production of various products such as enzymes, antibodies, organic acids, vaccines, etc. is facilitated and done on a large scale. The desired microorganisms are grown under controlled, sterile and suitable conditions for this purpose.

Explore more: Process Of Biotechnology

Here we will discuss the principles of biotechnology.

Principles of Biotechnology

Modern biotechnology is highly dependent on genetic engineering.

Genetic Engineering

The principle of genetic engineering is to manipulate and modify the genetic material of an organism or plants to insert desirable traits. Recombinant DNA technology is the main pillar of genetic engineering.

Recombinant DNA Technology is a technique to alter genes of an organism or plant. The desired gene is inserted into host using recombinant DNA technology. The host shows the desired trait phenotypically, which is governed by the inserted gene.

The recombinant DNA technology involves following main steps:

  • Selection of the desired gene
  • Selection of vector for the transfer of the gene known as a cloning vector, e.g. plasmid
  • Insertion of recombinant DNA into the host
  • Maintaining the introduced DNA in the host so that it is passed on to the next generation

Recombinant DNA Technology requires various tools like vector, host and enzymes such as restriction enzymes, ligases, polymerases, etc.


  • Restriction enzymes are known as molecular scissors, that cut the desired sequence of DNA.
  • This DNA is then ligated into the vector with the help of ligases before inserting it into the host organism.
  • The DNA-vector combination is known as the Recombinant DNA which is then transformed into the host.
  • This recombinant DNA, also known as the foreign DNA gets multiplied within the host.
  • It is then provided with optimum conditions to induce the expression of the target protein. This protein is known as the recombinant protein.
  • Many genetically modified crops are produced using this technology, e.g. Bt cotton a pest-resistant variety of cotton.

Bioprocess Engineering

Modern biotechnology is responsible for the advancement in the pharmaceutical industry. It helped in the production and storage of products like antibiotics, enzymes, vaccines, etc at a large scale.

A large amount of culture can be obtained by carrying out multiplication of organisms in the bioreactors under sterile and optimum conditions. We get a higher yield of the required product using bioprocess engineering.

Also Refer: Bioreactors


  • The obtained products are subjected to a series of processes before being marketed.
  • The products are purified by a process called downstream processing and formulated by undergoing clinical trials.
  • The product undergoes a strict quality check before it is subjected to further trials.

The modern processes in biotechnology are used for human welfare and have a significant impact on our life. The products have greatly enhanced various medicines and food production. Extensive research is going on this field to combat various diseases and improve quality of life.

Also Read: Applications Of Biotechnology in Medicine

Learn more in detail about Biotechnology, its principles, process, and other related topics at BYJU’S Biology

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