pH of Samples

The nature of the chemicals used in laboratories is either basic, acidic or neutral. This characteristic depends on the ions they release. A chemical is said to be acidic if it releases H+ ions in its aqueous solutions. A chemical is said to be basic if it releases OH ions in its aqueous solutions. In this experiment, we will learn how to find the pH of acids and bases.

Table of Contents


To determine the pH of the given samples using pH paper or universal indicator. The samples whose pH has to be determined are-

  1. Dilute CH3COOH
  2. Dilute NaOH
  3. Salt NaCl
  4. Dilute NaHCO3
  5. Water
  6. Lemon juice

Materials required:

  1. Six test tubes
  2. Test tube stand
  3. Dilute acid CH3COOH
  4. Dilute base NaOH
  5. Salt NaCl (preparation: dissolve 1 gram salt in 10 mL distilled water)
  6. Water
  7. Lemon juice
  8. Dilute NaHCO3
  9. Glass rod
  10. Measuring cylinder (10 mL)
  11. Standard pH colour chart
  12. pH paper
  13. Glass rod
  14. Dropper
  15. Universal indicator


What is pH?

pH is a measure of hydrogen ion concentration to determine the alkalinity or acidity of a solution.

  • If the pH value of a solution is less than 7 it is an acidic solution
  • If the pH value of a solution is greater than 7 it is a basic solution
  • If the pH value of a solution is equal to 7 it is a neutral solution

What is pH scale?

The pH scale consists of values which range from 0 (very acidic) to 14 (very alkaline). The numbers on the scale help to determine the hydrogen ion concentration.

What is pH paper?

pH paper can help us to know if a solution is basic, acidic or neutral. When the pH paper is dipped into a solution whose pH has to be determined, a colour will be developed. This colour is compared with the standard pH colour chart. Instead of pH paper, we can also use universal indicator paper or universal indicator solution.

What is universal indicator?

A universal indicator is a mix of pH indicator solutions that are designed to determine the pH of solutions over a wide range of values. Put a drop of solution on the universal pH indicator paper. The colour developed on the paper is matched with the standard pH colour chart.

pH value chart:

pH value chart

Also Read: pH of Samples Viva Questions

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Importance of pH in Daily Life

Experimental Setup:

pH of samples
Testing pH using universal indicator paper


  1. Wash six test tubes with distilled water and put them on test tube stand and label them A, B, C, D, E, F.
  2. Add 2ml of CH3COOH in test tube A, Add 2ml of NaOH in test tube B, Add 2ml of NaCl in test tube C, Add 2ml of NaHCO3 in test tube D, Add 2ml of Water in test tube E, Add 2ml of Lemon juice in test tube F.
  3. Take white tile, place 6 pH paper and label them A, B, C, D, E, F.
  4. Use a dropper or glass rod to put the respective sample solutions on the labelled pH paper placed on the white tile.
  5. Observe the colour change.


Sample Colour on pH paper
A Orange
B Dark blue
C Green
D Light blue
E Green
F Pink

Result and Conclusion:

Test tube Solution pH colour paper pH Nature
Sample A CH3COOH Orange 3 Weak acid
Sample B NaOH Dark blue 14 Strong base
Sample C NaCl Green 7 Neutral
Sample D NaHCO3 Light blue 9 Weak base
Sample E Water Green 7 Neutral
Sample F Lemon juice Pink 2 Weak acid

Precautions to be taken during the experiment:

  • Use freshly prepared test sample for the experiment.
  • The fruit juice sample should also be fresh to get the proper pH values.
  • Glass rod or dropper used for one sample should be washed thoroughly before using it for the other samples.

Viva Voce:

  1. Who was the first scientist to introduce pH?
    Ans: Soren Peder Lauritz Sorensen a Danish chemist.
  2. Define the pH of a solution.
    Ans: It is a measure of the basic or acidic power of a solution. It is a scale to measure the hydrogen ion concentration in the solution. Lower the hydrogen ion concentration higher is the pH.
  3. What is the pH of pure water at 25 °C?
    Ans: 7.
  4. There are two solutions with different pH values. Solution A has a pH value 6 and solution B has a pH value 5. Which solution is more acidic?
    Ans: Solution B whose pH value is 5 is more acidic.
  5. If the pH value of a solution is 9 then what is its colour?
    Ans: Light blue.



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  1. what is ph value?

  2. 4th ques the answer is SOLUTION B
    Can you tell me why?

  3. pH of nacl is 1?

    • The pH of a sodium chloride solution remains ≈7 due to the extremely weak basicity of the Cl− ion, which is the conjugate base of the strong acid HCl.