Bromothymol Blue - C<sub>27</sub>H<sub>28</sub>Br<sub>2</sub>O<sub>5</sub>S

What is Bromothymol blue?

Bromothymol Blue is an indicator in the pH range from 6.0 to 7.6. Bromothymol blue is the most commonly used pH indicator and is in low concentration and size container and low toxicity. It contains a pH indicator, bromothymol blue, which causes the medium to change from yellow to blue green; it contains antimicrobial agents, chloramphenicol and chlortetracycline, and an antimycotic agent, cycloheximide.

Other name – Dibromothymolsulfophthalein, 3,3′-Dibromothymolsulfonphthalein

C27H28Br2O5S

Bromothymol Blue

Density

1.25 g/cm³

Molecular Weight/ Molar Mass

624.384 g/mol

Boiling Point

614.26° C at 760 mmHg

Melting Point

202 °C

Chemical Formula

C27H28Br2O5S

Bromothymol blue Structure – C27H28Br2O5S

Bromothymol Blue

Physical Properties of Bromothymol blue – C27H28Br2O5S

Odour

Odourless

Appearance

Blue in basic solutions, yellow in acidic solutions and green in neutral solution

Covalently-Bonded Unit

1

Complexity

818

pH

7.6

Solubility

Sparingly soluble in water

Uses of Bromothymol blue – C27H28Br2O5S

  • In conjunction with phenol red, bromothymol blue was used to monitor the activity of the fungal asparaginase enzyme with phenol turns to pink and bromothymol blue turns blue indicating an increase in pH and thus enzyme activity.
  • In obstetrics, bromothymol is used to detect premature membrane rupture. Typically, amniotic fluid has a pH > 7.2, so bromothymol turns blue when it comes into contact with amnion – leaking fluid.

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