Paramecium or Paramoecium is a genus of unicellular ciliated protozoa. They are characterised by the presence of thousands of cilia covering their body. They are found in freshwater, marine and brackish water. They are also found attached to the surface. They are slipper-shaped and reproduce sexually by conjugation. They are easy to cultivate and widely used to study biological processes.
Paramecium are unicellular and eukaryotic, so they are kept in the kingdom Protista. They are ciliated protozoan and come under phylum Ciliophora.
The common species of Paramecium include:
- The cell size varies from 50 µ to 300 µ. The cell is ovoid, slipper or cigar-shaped
- The cellular cytoplasm is enclosed in a pellicle. Pellicle consists of an outer plasma membrane, inner epiplasm and a layer of alveoli, present in between both the layers. The pellicle is elastic and gives the cell its definite but changeable shape
- Cilia project from the depressions in the pellicle and cover the entire body surface. They are used for locomotion and taking nutrient-rich water inside the gullet
- Protoplasm is divided into outer ectoplasm and inner endoplasm, which is granular
- Trichocysts are present embedded in the ectoplasm. They are a defensive organ
- The endoplasmic granules reserve food. Some of the granules are secretory or excretory
- Paramecia contain at least two nuclei, micronuclei (one or more) and one macronucleus. Micronuclei have diploid chromosomes and take part in the reproduction. Macronuclei regulate all vital metabolic activities and growth. The macronucleus has multiple copies of the genome, i.e. polyploid
- Contractile vacuoles are present and their number varies from species to species. They are required for osmoregulation and expel the additional absorbed water
- At the midpoint, there is an oral groove on the ventral side known as the vestibule. Food is drawn inside the cell due to coordinated movement of cilia
- The oral groove opens in the mouth known as cytostome and to the gullet or pharynx
- There are numerous food vacuoles present for digesting food
- There is an anal pore present on the ventral surface in the posterior half of the cell known as cytoproct or cytopyge, which helps in egesting undigested food
The coordinated movement of thousands of cilia propels paramecium. Paramecium can rotate around its axis and move in the reverse direction on encountering an obstacle.
They are mostly heterotrophic. They feed on bacteria, algae, yeast and other microorganisms. They are holozoic. The food-laden water is drawn inside by the movement of cilia and it goes to the cytostome and to the gullet (cytopharynx).
The food gets loaded at the posterior end of cytopharynx. It gets surrounded by vacuoles, pinches off and circulates in the endoplasm. The food is acted upon by digestive enzymes present in the food vacuoles.
The undigested residue is egested through the temporary anal pore (cytopyge).
Some of the Paramecium species, e.g. Paramecium bursaria, etc. form a symbiotic relationship with green algae. Algae are present as an endosymbiont and provide food to paramecium by photosynthesis, in turn, the algae get a safe and protective habitat.
Paramecium may have intracellular bacteria known as kappa particles. Paramecium with kappa particles have the ability to kill other strains of paramecium.
Asexual Reproduction in paramecium is by binary fission. The mature cell divides into two cells and each grows rapidly and develops into a new organism. Under favourable conditions, Paramecium multiply rapidly up to three times a day. Binary fission divides a cell transversely and followed by mitotic division in the micronucleus. Macronucleus divides amitotically. The gullet also divides into two halves.
Although the favoured mode of reproduction in Paramecium is asexual, but they reproduce sexually too, when there is a scarcity of food.
Sexual reproduction in Paramecium is by various methods.
In conjugation, two complementary paramecia (syngen) come together and there is a transfer of genetic material. An individual has to multiply asexually 50 times before reproducing by conjugation.
In the process of conjugation, the conjugation bridge is formed and united paramecia are known as conjugants. Macronuclei of both the cells disappear. The micronucleus of each conjugant forms 4 haploid nuclei by meiosis. Three of the nuclei degenerate. The haploid nuclei of each conjugant then fuse together to form diploid micronuclei and cross-fertilization takes place. The conjugants separate to form exconjugants. They are identical but different from the earlier cells. Each exconjugate undergoes further division and forms 4 daughter Paramecia. Micronuclei form a new macronucleus.
Paramecium also show autogamy i.e. self-fertilization. A new macronucleus is produced, which increases their vitality and rejuvenates them.
Cytogamy is less frequent. In cytogamy, two paramecia come in contact but there is no nuclear exchange. Paramecium rejuvenates and a new macronucleus is formed.
A Paramecia undergoes ageing and dies after 100-200 cycles of fission if they do not undergo conjugation. The macronucleus is responsible for clonal ageing. It is due to the DNA damage.